NSTRUCTORS: Dr.Len Holmes/Dr. Siva Mandjiny

TEXT: Instructional materials will be provided.

DESCRIPTION: This is a laboratory intensive course designed to train students in selected aspects of separation/purification techniques of protein and analytical methods.

CONTENT:

1. Introduction to Bioprocessing and the need for bioseparation

2. Protein sources and downstream processing

3. Protein separation/purification techniques

- making an extract

- protein/enzyme assay techniques

- separation by precipitation

- dialysis

- chromatography techniques includes

A) Adsorption

B) Ion exchange

C) Affinity

D) Gel filtration

E) HPLC

4. Membrane separation methods

- filtration methods

- affinity chromatography

5. Electrophoretic methods

- analytical

- preparative

6. Two phase partitioning

- cells

- proteins

7. other useful techniques for the separation of protein