THE UNIVERSITY OF NORTH CAROLINA AT PEMBROKE
DEPARTMENT OF CHEMISTRY AND PHYSICS
MOLECULAR BIOTECHNOLOGY LABORATORY
BIOANALYTICAL CHEMISTRY (4 credits)
CHM 331 (FALL 1997)
TUESDAY AND Thursday 11:00AM TO 1:45PM
INSTRUCTOR: Dr. Siva Mandjiny
Phone: 910 521 6650/6608 (office) 910 521 4752 (Home)
E-mail: mandjini@nat.pembroke
DESCRIPTION: This is a laboratory-intensive course designed to train students in selected aspects of bioanalytical chemistry. The laboratory will focus on analysis of fermentation products, enzyme kinetics, protein purifications and separation methods.
LIST OF PROJECTS
|
PROJECT # |
DESCRIPTION |
|
1 |
General lab techniques |
|
2 |
Basic calculations |
|
3 |
Protein Assay: Biuret, Lowery and Bradford Tests |
|
4 |
Enzyme assay |
|
5 |
Separation Techniques: 1. Gel filtration |
|
6 |
2: Ion exchange and Affinity Chromatography |
|
7 |
3: HPLC (High Performance Liquid Chromatography) |
|
8 |
Electrophoresis analytical and preparative |