Biology

Faculty Research

Faculty Research

Andy Ash PhotoDr. Andy Ash: “Amphibian Biology”
The primary focus of my research is the effects of forest management techniques on populations of terrestrial salamanders in the southern Appalachian Mountains. It turns out these terrestrial salamanders are great indicators of the ecological health of forest litter, therefore helping to predict the return of ecological function to forests after tree harvest. Student opportunities: Fall/Spring (1) Summer (1)

 

Ben Bahr PhotoDr. Ben Bahr: “Therapeutic Treatments Against the Numerous Diseases that Disrupt Brain Function”
Alzheimer's Disease Research Lab (PDF brochure)

The 100,000 Gbyte hard-drive we call our brain is a challenge to study, also making it a challenge to find therapeutic treatments against the numerous diseases that disrupt brain function. In my lab, slices of brain tissue are kept alive to examine neuronal connections responsible for both memory encoding and cellular maintenance pathways, and to study their vulnerability to pathogenesis. While the brain’s incredible density of synaptic connections allows for extraordinary memory capacity, the abundant synapses are also vulnerable to pathogenic over-activation. Such excitotoxic brain damage can occur in many disease states including stroke, traumatic injury, and seizure events. We are studying the pharmacological enhancement of endogenous pathways, and we found that positive modulation of internal repair mechanisms protects against the damaging effects of seizures and stroke-type excitotoxic insults. Other efforts are to study age-related neurodegenerative disorders. Every 72 seconds someone in the U.S. develops Alzheimer’s disease (AD). Reducing Alzheimer-type protein accumulation is essential for slowing the progression of the disease. Lysosomes and their degradative enzymes (e.g. cathepsins) are known to respond to AD, perhaps in an attempt to offset the abnormal protein accumulations that cause a distinct pathogenic cascade. Recently, we discovered a new class of drugs that act as positive modulators of the lysosomal response, resulting in the up-regulation of cathepsins as well as neuroprotection in cultured brain slices and in mouse models of AD.

Dr. Wm. Bruce EzellDr. Wm. Bruce Ezell, Jr.: “Entomology”
My research concerns field studies of insects and their behavior patterns. I am interested in the host seeking behavior of deer flies and horse flies (Diptera: Tabanidae). I am curious as to exactly how these “micro-predators” locate us in order to seek our blood. Some of my other studies have centered around the effects of color and various hues and how they are perceived by these flies. I am also interested in the effects of dredging and dredged material disposal, as these procedures greatly enhance larval mosquito development. Dredge material disposal creates square miles of a man-made environment. I am also concerned with the use of weathering soil deposit patterns as indicators and predictors of mosquito oviposition activity.

Dr. Debby HanmerDr. Deborah Hanmer: “Botany”
My training is in Plant Pathology. One current research project involves the interactions between a soil fungus and nematodes on tomato plants. Another interest of mine involves black soils from the Amazon call terra preta. These fertile soils seem to be created using charred organic matter, and I have two students working on projects to determine the viability of using “biochar” to enhance the fertility of local soils. 
Student opportunities: Fall/Spring (2) Summer (TBD)

 

Dr. Leon JerniganDr. Leon Jernigan: “Environmental Biology”
Research area involves coastal marshes and freshwater wetlands. Topics of interest are hydrologic alterations, success of restoration/ mitigation projects, and environmental monitoring. Much of the expertise obtained by involved students would be in the area of applied ecology. Student opportunities: Fall/Spring (1) Summer (1)

Lisa Kelly PhotoDr. Lisa Kelly: “Conservation Biology”
I’m a field biologist with research interests in the complementary fields of ecology, conservation biology, and plant taxonomy. More specifically, floristics, rare species, and the effects of natural and anthropogenic disturbances on diversity, interest me. As the director of the UNCP Herbarium, I have supervised and trained several students in standard herbarium techniques. I look forward to expanding the Herbarium’s holdings and assisting students who are interested in the collection and preservation of voucher specimens. I have directed undergraduate research on Chrysoma pauciflosculosa (woody goldenrod), a state endangered species of plant. The work focused on population dispersion in the field and seed germination in the laboratory. Of late, I have taken an interest in the population dynamics and stable isotopes of Solenopsis invicta (invasive fire ant) in Carolina bays. 
Student opportunities: Fall/Spring (1) Summer (1)

Prof. Brandi NormanBrandi Norman: "Amphibians as Indicators of Environmental Changes" 
My research interests are to continue the salamander research I was working on in southwest Virginia before coming to UNCP.  This research focuses on global warming and it's impacts on salamander species in SW Virginia. Amphibians are excellent indicators of environmental changes, such as climate, and are susceptible to UV radiation and chitrid fungus, which can quickly eliminate a small population.  The goal of this research is to gain data on the temperature changes occurring at different elevations along White Top Mountain, on the impacts and/or migrations of salamander species in response to temperature changes, and to look for decreases in the numbers of endangered and rare species, such as the Weller Salamander (Plethodon welleri), that live only in that one area of the world. This research is conducted each spring and is planned to be a 10-year study and has been ongoing for the past four years.  It would be great to extend the research into North Carolina in the future.

Dr. Maria PereiraDr. Maria Pereira: “Molecular Genetics, Biotechnology”
I have had three different projects in which students participated. The first dealt with biofuels and the production of biodiesel. This project was performed in collaboration with Drs. Siva Mandjiny and Tom Dooling. UNCP owns a reactor in which kitchen oil can be converted into biodiesel. We have been looking at ways to use enzymes instead of the standard chemicals to make the reaction more efficient. One of the by-products of the reaction is glycerin. We need to find a use for the glycerin. Students have had the opportunity to use their creative thinking, which is usually called "novel" research. The second project involved the growth and expression of eukaryotic cells and was done in collaboration with Dr. Jeremy Sellers. Human pancreatic cells (cancerous and control) were evaluated and maintained alive in a CO2 incubator. We explored different cell lines and looked for the mutations present. Techniques used included PCR, DNA sequencer, bioinformatics and cloning. The third project involved bacterial mutants. I have created E. coli mutants that are ampicillin resistant (no pAMP present). We evaluated these mutants and compared and contrasted them for their physiological and biological characteristics. We searched for the mutation present in each one by using a DNA sequencer, and we compared this mutation against all other known mutants by using bioinformatics. Student opportunities: None for the 2010-2011 Academic year

Dr. Robert PoageDr. Robert Poage: “Neurobiology, Synaptic Physiology”
Synaptic transmission is the basis of all movement, sensation, and stimulation. My research involves ion channel function, primarily voltage-activated calcium channels. I am interested in specialization of neuronal membrane and how certain cellular compartments are capable of extremely rapid interneuronal communication. My primary training is in the use of electrophysiological recording techniques to study basic properties of neuronal communication. I am currently investigating the role of the presynaptic environment on voltage-gated calcium channel function using the frog neuromuscular junction as a model system. We are currently addressing the following hypotheses: 1st, that DAP increases overall acetylcholine release while decreasing the effects of facilitation in LEMS conditions; 2nd, that DAP increases facilitation in LEMS conditions compared to control conditions. 
Student opportunities: Fall/Spring (1) Summer (2)

Dr. John RoeDr. John Roe: "Wildlife Ecology"
Research in my lab focuses on wildlife behavior, population biology, and ecology. Students that work with me will log many hours in the field using technologies to monitor populations and examine behavioral responses to environmental challenges such as climate change, habitat modification, roads, and pollution. Technologies that students will have the opportunity to use include radio-telemetry, global positioning systems, environmental data loggers, geographic information systems, automated recording devices, and others. I am particularly interested in reptiles and amphibians in the Lumber River and associated wetlands, the sandhills, and the unique depression wetlands known as Carolina bays. I conduct basic descriptive research, but I also think it is important to apply research findings to addressing specific wildlife conservation and management issues.

Dr. Maria SantistebanDr. Maria Santisteban: “Yeast Molecular Genetics, Epigenetic Regulation of Eukaryotic Gene Expression”
In the eukaryotic cell nucleus, DNA is packaged with histones into nucleosomes, the repeating subunits of chromatin. The precise organization of DNA in chromatin has important functional consequences. DNA-template processes such as transcription, replication and chromosome segregation are dependent on the remarkable packaging of the DNA in chromatin. The long-range objective of my research is to understand the molecular mechanisms of chromatin-regulated gene activation. My research has primarily focused on the role of a histone H2A variant, the H2A.Z/F. In the budding yeastSaccharomyces cerevisiae, this variant histone protein is encoded by the HTZ1 gene. We have discovered that Htz1 functions to enable transcription by RNA polII. Specifically, our data indicate that Htz1 possibly plays a role in elongation. I would continue exploring this hypothesis using techniques of molecular genetics that will involve mutational analysis, genetic screens, chromatin immunoprecipitation, microarray analysis, Real-time PCR, etc. 
Student opportunities: Fall/Spring (2) Summer (2)

Dr. Marilus SantosDr. Marilu Santos: “Microbiology, Public Health and Microbial Genetics”
My research interest is focused on using the techniques of microbial and molecular genetics to explore fundamental processes in microbial cells, such as morphogenesis, pathogenesis, thermotolerance, metabolite production, and antibiotic resistance; biotechnology activities including development of strains for bioprospecting using molecular tools and public health related activities along the line of antimicrobial testing and establishing incidence of common pathogens in food, water, industrial products and relevant public places. 
Student opportunities: Fall/Spring (2) Summer (2)

Jeremy Sellers PhotoDr. Jeremy Sellers: “Lipid Biosynthesis, Immunology”
My research interests lie in developing a better understanding of lipid transport systems and the biogenesis of large lipid transfer proteins. We have recently shown that an ortholog of a factor involved in the formation of mammalian atherosclerotic lipoproteins is expressed in invertebrates. I wish to continue to explore the importance of this co-factor in invertebrate lipid transport systems and potentially relate this to the biogenesis of human atherosclerotic lipoproteins. Such research exposes students to multiple techniques in molecular and cellular biology as well as promoting an understanding of the basics of protein trafficking and the use of bioinformatics in research. 
Student opportunities: Fall/Spring (2) Summer (2)

Dr. Patricia SellersDr. Patricia Sellers: “Environmental Biology and Chemistry, Lake/River Ecology, Water Quality Testing”
Research interests included metal and organic contaminants in lakes and rivers, surface water quality, eutrophication, indicator species of water quality, land-use (example urbanization) affecting water quality, and microbial regulation of water chemistry.  Current work deals with historical but active mercury contamination in the traditional territories of several First Nations in Canada. 
Dr. Sellers is an Assistant Professor in the Department of Biology and is the part-time Executive Director of The Lumber River Conservancy
Student opportunities: Fall/Spring (1) Summer (1)

Dr. Velinda WoriaxDr. Velinda Woriax: “Molecular Biology”
As a biochemist, my research interest includes a variety of topics: protein/protein interactions, enzymology, protein signaling, health issues of Native Americans, and science education among K-12. Currently at UNCP, our laboratory has begun a pilot study of isozymes found in a local evergreen shrub called Chrysoma pauciflosculosa. Undergraduate students are developing protocols to examine several isozymes of these populations for comparison between sites and within each population. Methods involved include electrophoresis using native acrylamide gels (native PAGE) and starch gel electrophoresis and molecular biology techniques. A second project involves the use of herbal extracts for antimicrobial therapy. Thus far, combinatorial and selected temperature and pH effects have been addressed with a student now attempting to determine effects on the bacterial morphology. It remains to be elucidated the thermostability of the extracts. Student opportunities: Fall/Spring (2) Summer (2)